Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: List of primers used in qRT-PCR.

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 (a) and MyD88 (b) mRNA expression during prevention of IND‐induced duodenal ulceration. Levels of TLR-2 and MyD88 mRNA were determined by qRT-PCR assay. β -Actin mRNA was used as internal control for equal loading. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 and MyD88 protein expression during prevention of IND induced duodenal ulceration. (a) Representative immunoblots for TLR-2 and MyD88 proteins as measured by western blot analysis using specific antibodies. Loading control was monitored by β -actin immunoblotting. Quantitative analysis derived from densitometric scans of western blots of TLR-2 (b) and MyD88 (c) proteins of duodenal mucosa. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Western Blot, Derivative Assay, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Localization of TLR-2 and MyD88 in duodenal tissues after IND administration and effect of LZD thereon. (a) Representative pictures showing the immunohistochemical analysis of TLR-2 and MyD88 in sections of duodenal mucosa obtained from rats in normal control, negative control, ESO, and LZD groups. The black arrows denote the immunopositive expression parts of TLR-2 and MyD88. Magnification 200x. (b, c) Quantitative analysis of the immunohistochemical signals as measured by IOD values. Results are expressed as the mean ± SEM ( n = 3). Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Immunohistochemical staining, Negative Control, Expressing